Journal: Biomaterials Research
Article Title: Hollow Bismuth Nanoparticle-Loaded Gelatin Hydrogel Regulates M2 Polarization of Macrophages to Promote Infected Wound Healing
doi: 10.34133/bmr.0105
Figure Lengend Snippet: Evaluation of the effect of GO-PS/HAL@Bi hydrogel on HaCaT cells and RAW 264.7 cells. (A) Cell viability of HaCaT cells treated with hydrogel extract for 24 h detected by the CCK-8 method ( n = 4, * P < 0.05, ** P < 0.01). (B) Live/dead staining of HaCaT cells treated with hydrogel extract for 24 h. (C) Cytoskeleton staining of HaCaT cells treated with hydrogel extract for 24 h. (D) Cumulative release rate curves of HAL from hollow Bi and GO-PS/HAL@Bi hydrogel. (E) Quantitative data of iNOS and CD206 expression obtained by flow cytometry analysis of LPS-induced RAW 264.7 cells treated with GO-PS, GO-PS/Bi, or GO-PS/HAL@Bi ( n = 3, * P < 0.05). (F) Representative immunofluorescence images of LPS-induced RAW 264.7 treated with GO-PS, GO-PS/Bi, or GO-PS/HAL@Bi hydrogel.
Article Snippet: CD206 polyclonal antibody and inducible nitric oxide synthase (iNOS) polyclonal antibody were purchased from Proteintech (Wuhan, China).
Techniques: CCK-8 Assay, Staining, Expressing, Flow Cytometry, Immunofluorescence